The Effect of Temperature on the Enzyme Catalase ..

 I am investigating the effect of the concentration of enzyme on the rate of reaction.

Investigating the effect of pH on the activity of the enzyme catalase

The different concentrations will be: 0.5% 0.75% 1.0%
1.5% 2%

An enzyme is a class of protein, which acts as a biological catalyst
to speed up the rate of reaction with its substrates.

Graph 4- the effect of increase in substrate concentration of the rate of an enzyme-catalysed reaction.

Effect of the catalase heme concentration on the titration curve.



Introduction: I am investigating the effect of the concentration of
the enzyme, amylase on the time taken for the enzyme to fully
breakdown the substrate, to a sugar solution.

(1)Todetermine the effect of substrate concentration on enzyme activity, obtaineight 50 ml beakers and label them as follows:

DISCUSSION: Mitochondrial abnormalities increase the production of free radicals and reported as a potent culprit for the pathogenesis of maternally inherited type 2 diabetes mellitus 14. Hitherto, prodigious work on mitochondrial DNA mutations suggested that, mutated mitochondria inherited in the families via mother, meaning, patients with a history of maternal inheritance of type 2 diabetes mellitus are more prone to free radical attack 9, 32, 33. Furthermore, hyperglycemia in diabetes mellitus could increase free radical concentration, which exerts deleterious effect on cells and organs and result in other serious complications like, cardiovascular diseases, atherosclerosis and aging 34, 35. In the past few decades, research on diabetes mellitus was strictly focused on insulin related consequences and tended to look at the alterations in the concentration of free radicals and its scavenging system along with the levels of lipid profile parameters 9. Recently, much prominence has been placed on the correlation studies between enzymatic antioxidants, HbA1c, lipid peroxidation and lipid profile parameters 30. The present study was undertaken to evaluate the status of oxidative stress and lipid profile parameters including their correlation studies with lipid peroxidation in patients with maternally inherited type 2 diabetes mellitus.